High-Resolution Melting Publications
Contact us for reprints v.03/12/08
Basics of the Technology
  1. Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem. 2003 Jun; 49(6):853-60.

  2. Liew M, Pryor R, Palais R, Meadows C, Erali M, Lyon E, Wittwer C. Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. Clin Chem. 2004 Jul; 50(7):1156-64.

  3. Zhou L, Myers AN, Vandersteen JG, Wang L, Wittwer CT. Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye. Clin Chem. 2004 Aug; 50(8):1328-35. Epub 2004 May 27./ editorial.

  4. Reed GH, Wittwer CT. Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clin Chem. 2004 Oct; 50(10):1748-54. Epub 2004 Aug 12.

  5. Zhou L., Wang L., Palais R., Pryor R. and Wittwer CT. High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution. Clin Chem. 2005 Oct; 51(10): 1770-7. / editorial.

  1. Highsmith WE Jr. SNPs for sale. Cheap! Clin Chem. 2004 Aug; 50(8):1296-8.

  2. von Ahsen N. Two for Typing: Homogeneous combined single-nucleotide polymorphism scanning and genotyping. Clin Chem. 2005 Oct; 51(10):1761-2.

Technology Comparisons

  1. Herrmann MG, Durtschi JD, Wittwer CT, Voelkerding KV.  Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping.  Clin Chem. 2007 Jun 7; [Epub ahead of print].

  2. Herrmann MG, Durtschi JD, Bromley LK, Wittwer CT, Voelkerding KV.  Instrument comparison for heterozygote scanning of single and double heterozygotes: a correction and extension of Herrmann et al., Clin Chem 2006;52:494-503Clin Chem. 2007;53:150-2.

  3. Liew M, Seipp M, Durtschi J, Margraf RL, Dames S, Erali M, Voelkerding K, Wittwer C.  Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting. Am J Clin Pathol. 2007;127:341-8.

  4. Herrmann MG, Durtschi JD, Bromley LK, Wittwer CT, Voelkerding KV. Amplicon DNA Melting Analysis for Mutation Scanning and Genotyping: Cross-Platform Comparison of Instruments and Dyes. Clin Chem. 2006 Mar; 52(3)::494-503. Epub 2006 Jan 19.

  5. Chou LS,Lyon E, Wittwer CT. A comparison of high-resolution melting analysis to denaturing high performance liquid chromatography for mutation scanning: cystic fibrosis transmembrane conductance regulator gene as a model. Am J Clin Pathol, 2005 Sep; 124(3):330-8.

Advanced Techniques

  1. Seipp MT, Durtschi JD, Liew MA, Williams J, Damjanovich K, Pont-Kingdon G, Lyon E, Voelkerding KV, Wittwer CT.  Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting.  J Mol Diagn. 2007 Apr 26; [Epub ahead of print].

  2. Dames S, Margraf RL, Pattison DC, Wittwer CT, Voelkerding KV. Characterization of aberrant melting peaks in unlabeled probe assays.  J Mol Diagn. 2007 Apr 26; [Epub ahead of print].

  3. Margraf RL, Mao R, Wittwer CT. Masking selected sequence variation by incorporating mismatches into melting analysis probes. Hum Mutat. 2006 Mar; 27(3)::269-278. Epub 2006 Jan 12.

  4. Palais RA, Liew M, Wittwer CT. Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping. Anal Biochem. 2005 Nov; 346(1):167-75. Epub 2005 Aug 31.

  5. Graham R, Liew M, Meadows C, Lyon E, Wittwer CT. Distinguishing different DNA heterozygotes by high-resolution melting.Clin Chem. 2005 Jul; 51(7):1295-8.

  6. Gundry CN, Vandersteen JG, Reed GH, Pryor RJ, Chen J, Wittwer CT: Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes. Clin Chem. 2003;49: 396-406.

Oncology (heterozygous dominant mutations & allele fractions)

  1. Margraf RL, Mao R, Wittwer CT. Rapid Diagnosis of MEN2B Using Unlabeled Probe Melting Analysis and the LightCycler 480 Instrument. J Mol Diagn. 2008 Mar;10(2):123-8.

  2. Willmore-Payne C, Holden JA, Chadwick BE, Layfield LJ. Detection of c-kit exons 11- and 17-activating mutations in testicular seminomas by high-resolution melting amplicon analysis. Mod Path. 2006 Jun 2 [Epub ahead of print].

  3. Margraf RL, Mao R, Highsmith WE, Holtegaard LM, Wittwer CT.  RET proto-oncogene genotyping using unlabeled probes, the masking technique, and amplicon high-resolution melting analysis. J Mol Diagn. 2007;9:184-96.

  4. Willmore-Payne C, Volmar KE, Huening MA, Holden JA, Layfield LJ.  Molecular diagnostic testing as an adjunct to morphologic evaluation of pancreatic ductal system brushings: potential augmentation for diagnostic sensitivity.  Diagn Cytopathol. 2007;35:218-24.

  5. Nomoto K, Tsuta K, Takano T, et al.: Detection of EGFR mutations in archived cytologic specimens of non-small cell lung cancer using high-resolution melting analysis. Am J Clin Pathol 2006;126:608-615.

  6. Willmore-Payne C , Holden JA, Layfield LJ. Detection of EGFR and HER2 activating mutations in lung adenocarcinoma by high resolution melting amplicon analysis: correlation with gene copy number, protein expression and hormone receptor expression. Human Path. 2006 Jun; 37(6): 755-763.

  7. Willmore-Payne C, Holden JA, Layfield LJ. Detection of EGFR and HER2 activating mutations in squamous cell carcinoma involving the head and neck Modern Path. 2006 May; 19(5): 634-640.

  8. Margraf RL, Mao R, Highsmith WE, Holtegaard LM, Wittwer CT. Mutation scanning of the RET protooncogene using high-resolution melting analysis. Clin Chem. 2006 Jan; 52(1):138-41.

  9. Willmore-Payne C, Layfield LJ, Holden JA. c-KIT mutation analysis for diagnosis of gastrointestinal stromal tumors in fine needle aspiration specimens. Cancer. 2005 Jun 25;105(3):165-70.

  10. Willmore-Payne C, Holden JA., Tripp S, Layfield LJ. Human malignant melanoma: Detection of BRAF- and c-kit-activating mutations by high-resolution amplicon melting analysis. Hum Pathol, 2005 May; 36(5):486-93.

  11. Willmore C, Holden JA, Zhou L, Tripp S, Wittwer CT, Layfield LJ. Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis. Am J ClinPathol. 2004 Aug; 122(2):206-16.

  12. Vaughn CP, Elenitoba-Johnson KS. High-resolution melting analysis for detection of internal tandem duplications. J Mol Diagn. 2004 Aug; 6(3):211-6.

Infectious Disease (haploid genomes)

  1. Dames S, Pattison DC, Bromley LK, Wittwer CT, Voelkerding KV. Unlabeled probes for the detection and typing of herpes simplex virus. Clin Chem. 2007 Oct; 53(10):1847-54.

  2. Slinger R, Bellfoy D, Desjardins M, Chan F: High-resolution melting assay for the detection of gyrA mutations causing quinolone resistance in Salmonella enterica serovars Typhi and Paratyphi. Diagn Microbiol Infect Dis 2007;57:455-458.

  3. Cheng JC, Huang CL, Lin CC, et al.: Rapid detection and identification of clinically important bacteria by high-resolution melting analysis after broad-range ribosomal RNA real-time PCR. Clin Chem 2006;52:1997-2004.

  4. Erali M, Pouner JI, Woods, GL, Petti CA, Wittwer CT.  Multiplex single-color PCR with amplicon melting analysis for identification of Aspergillus species. Clin Chem  2006;52:1443-5.

  5. Odell ID, Cloud JL, Seipp M, and Wittwer CT. Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting of hsp65 PCR products. Am J Clin Path 2005 Jan; 123(1):96-101.

HLA matching

Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais R, Wittwer CT. High-resolution DNA melting curve analysis to establish HLA genotypic identity. Tissue Antigens. 2004 Aug; 64(2):156-64.

Genotyping for genetic disorders

  1. Poulson MD, Wittwer CT. Closed-tube genotyping of apolipoprotein E by isolated-probe PCR with multiple unlabeled probes and high-resolution DNA melting analysis. Biotechniques. 2007 Jul;43(1):87-91.

  2. Seipp MT, Pattison D, Durtschi JD, Jama M, Voelkerding KV, Wittwer CT. Quadruplex genotyping of F5, F2, and MTHFR variants in a single closed tube by high-resolution amplicon melting. Clin Chem. 2008 Jan;54(1):108-15.

  3. Hill CE, Duncan A, Wirth D, Nolte FS. Detection and identification of cytochrome P-450 2C9 alleles *1, *2, and *3 by high-resolution melting curve analysis of PCR amplicons . Am J Clin Pathol . 2006 Apr; 125(4):584-591.

  4. Liew M, Nelson L, Margraf R, Mitchell S, Erali M, Mao R, Lyon E, Wittwer C. Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes . J Mol Diagn. 2006 Feb; 8(1):97-104.

Mutation scanning for genetic disorders

  1. Montgomery J, Wittwer CT, Kent JO, Zhou L. Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis. Clin Chem. 2007 Nov;53(11):1891-8.

  2. McKinney JT, Longo N, Hahn SH, Matern D, Rinaldo P, Strauss AW, Dobrowolski SF. Rapid, comprehensive screening of the human medium chain acyl-CoA dehydrogenase gene. Mol Genet Metab. 2004 Jun; 82(2):112-20.

  3. Dobrowolski SF, McKinney JT, Amatdi San Filippo C, GiakSim K, Wilcken B, Longo N. Validation of dye-binding/high-resolution thermal denaturation for the identification of mutations in the SLC22A5 gene. Hum Mutat. 2005 Mar; 25(3):306-13.

  4. Lonie L, Porter DE, Fraser M, et al.: Determination of the mutation spectrum of the EXT1/EXT2 genes in British Caucasian patients with multiple osteochondromas, and exclusion of six candidate genes in EXT negative cases. Hum Mutat 2006;27:1160.

  5. Kennerson ML, Warburton T, Nelis E, et al.: Mutation scanning the GJB1 gene with high-resolution melting analysis: implications for mutation scanning of genes for Charcot-Marie-Tooth disease. Clin Chem 2007;53:349-352.

  6. Dobrowolski SF, Ellingson C, Coyne T, Grey J, Martin R, Naylor EW, Koch R, Levy HL.  Mutations in the phenylalanine hydroxylase gene identified in 95 patients with phenylketonuria using novel systems of mutation scanning and specific genotyping based upon thermal melt profiles. Mol Genet Metab. 2007 Jul;91(3):218-27.

  7. Dobrowolski SF, E Ellingson C, Caldovic L, Tuchman M.  Streamlined assessment of gene variants by high resolution melt profiling utilizing the ornithine transcarbamylase gene as a model system. Hum Mutat. 2007 Jun 12; [Epub ahead of print].

  8. Vandersteen JG, Bayrak-Toydemir P, Palais RA, Wittwer CT.   Identifying common genetic variants by high-resolution melting.  Clin Chem. 2007 Jul;53(7):1191-8.

Reviews & Book Chapters
  1. Erali M, Wittwer CT. High resolution melting analysis for gene scanning. Methods 2010;50:250-61.

  2. Erali M, Voelkerding KV, Wittwer CT. High resolution melting applications for clinical laboratory medicine. Exp Mol Med.. 2008;85:50-8.

  3. Reed GH, Kent JO, Wittwer CT. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics. 2007 Jun;8(6):597-608.

  4. Montgomery J, Sandford L, and Wittwer CT. High-resolution DNA melting analysis in clinical research and diagnostics. Exp Rev of Mol Diag 10:219-40, 2010.

  5. McKinney JT, Nay LM, De Koeyer D, Reed GH, Wall M, Palais RA, Jarret RL, Wittwer CT. Mutation scanning and genotyping in plants by high resolution DNA melting. In: The Handbook of Plant Mutation Screening, Meksem K, Kahl G, eds. Wiley-VCH, Weinheim, 149-165, 2010.

  6. Farrar, JS, Reed GH, Wittwer CT. High resolution melting curve analysis for molecular diagnostics. In: Molecular Diagnostics, 2nd ed., Patrinos GP, Ansorge WJ, eds. Elsevier, London, Chapter 15, 229-245, 2010.

  7. Wittwer CT. High resolution DNA melting: advances and limitations. Hum Mutat 2009;30:857-859.

  8. Dujols, V., Kusukawa, N., McKinney, J.T., Dobrowolski, S.F., and Wittwer, C.T. (2006) High-resolution melting analysis for scanning and genotyping . In: Real-time PCR (Ed, Dorak, M.T.), pp. 155 – 169.Taylor & Francis Group, New York.

  9. Montgomery J, Wittwer CT, Palais R, Zhou L. Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis. Nature Protocols 2007:2;59-66.

  10. Erali, M, Palais, B, Wittwer, CT. (2007)  SNP genotyping by unlabeled probe melting analysis. In: Molecular Beacons – Signalling Nucleic Acid Probes, Methods and Protocols, Seitz O and Marx A, eds., Methods in Molecular Biology, Vol 429, (Seitz O, series editor), Humana Press, Totowa, New Jersey, pp. 199-206.