Rapid Cycle PCR Publications Contact us for reprints
Basics of the Technology
  1. Wittwer CT, GC Fillmore, DR Hillyard.  Automated polymerase chain reaction in capillary tubes with hot air.  Nucl. Acids Res. 17:4353-4357, 1989.

  2. Wittwer CT, GC Fillmore, DJ Garling.  Minimizing the time required for DNA amplification by efficient heat transfer to small samples.  Anal. Biochem. 186:328-331, 1990.

  3. Wittwer CT and DJ Garling.  Rapid Cycle DNA Amplification.  BioTechniques, 10:76-83, 1991.


Advanced Techniques

  1. Crews N, Wittwer C, Gale B. Continuous-flow thermal gradient PCR. Biomed Microdevices. 2008 Apr;10(2):187-95.

  2. Swerdlow H, BJ Jones, and CT Wittwer.  Fully automated DNA reaction and analysis in a fluidic capillary instrument.  Anal. Chem., 69:848-855, 1997.

  3. Heap DM, MG Herrmann, CT Wittwer.  PCR amplification using electrolytic resistance for heating and temperature monitoring.  BioTechniques, 1006-1012, 2000.

General Applications

  1. Segal GH, CT Wittwer, AJ Fishleder, MH Stoler, RA Tubbs, CR Kjeldsberg.  Identification of monoclonal B cell populations by rapid cycle PCR:  a practical screening method for the detection of immunogolbulin gene rearrangements.  Amer. J. Path., 141:1291-7, 1992.

  2. Weis JH, SS Tan, BK Martin, CT Wittwer.   Detection of rare mRNA species via quantitative RT-PCR.  Trends in Genetics, 8:263-4, 1992.

  3. Wittwer, CT, BC Marshall, GH Reed, JL Cherry.   Rapid cycle allele-specific amplification: studies with the cystic fibrosis delta F508 locus.  Clin Chem. 39:804-9, 1993.

  4. Molot RJ, TC Meeker, CT Wittwer, SL Perkins, GH Segal, AS Masih, RC Braylan, CR Kjeldsberg.  Antigen expression and PCR amplification of mantle cell lymphomas.  Blood, 83:1626-1631,1994.

  5. Hussey CE, E Lyon, A Millson, MJ Lay, CT Wittwer, and GH Segal.  A rapid, practical PCR-based approach for the detection of PML/RAR-alpha gene fusion in acute promyelocytic leukemia.  Am. J. Clin.

Reviews & Book Chapters
  1. Wittwer CT, GB Reed and KM Ririe.  Rapid cycle DNA amplification.  In K Mullis, F Ferre and R Gibbs (Eds.),  The polymerase chain reaction.  Springer-Verlag, Deerfield Beach, FL, 174-181, 1994.

  2. Brown RA, MJ Lay, and CT Wittwer.  Rapid cycle amplification for construction of competitive templates, in Genetic Engineering with PCR, (Horton, RM and RC Tait, eds.), Horizon Scientific Press, Norfolk, England, 57-70, 1998.

  3. Wittwer CT, Herrmann MG: Rapid thermal cycling and PCR kinetics, In: PCR Methods Manual. Innis M, Gelfand D, Sninsky J (Eds.), Academic Press, San Diego, 211-229, 1999.