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This portal delivers software, data science techniques and digital content exploring simple and rapid methods for nucleic acid analyses, with goals to provide better ways to expedite research and perform clinical diagnostic tests. For nearly three decades, the Wittwer Lab for DNA Analysis developed many innovative technologies now commonly used in research and clinical applications.
We continue to provide accessible and easy to use software that is free of registration and cost.
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Extreme PCR
PCR is a key technology in molecular diagnostics with an ability to amplify and quantify specific DNA fragments in less than an hour. Recently developed, Extreme PCR can be accomplished in 15-60 seconds and was developed while investigating the kinetic limits of PCR.
Learn More at Clinical Chemistry
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Detecting Copy Number Variation
Copy number alterations (CNAs), somatic changes to chromosome structure that result in gain or loss in copies of sections of DNA, are very common in cancer and associated with particular cancer types. It is possible to detect copy number variation with dNTP limited PCR and melt curve analysis.
Read More in Clinical Chemistry
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High-Speed Melting
Recent work details the effects of melting rate, amplicon size, and analysis method on small amplicon genotyping. Summarized in two publications, we conclude that faster rates improve melting analysis, just like faster rates improve PCR.
Read More About High-Speed Melting
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