Extreme PCR
PCR is a key technology in molecular diagnostics with an ability to amplify and quantify specific DNA fragments in less than an hour. Recently developed, Extreme PCR can be accomplished in 15-60 seconds and was developed while investigating the kinetic limits of PCR.
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High-Resolution Melting
High-density data acquisition and precision temperature control are used during melting so that PCR products (amplicons) can be genotyped, scanned for mutations, or matched for sequence identity directly after PCR. View Webinar

Instrument Comparison in Clinical Chemistry

Detecting Copy Number Variation
Copy number alterations (CNAs) - gains or losses in copies of sections of DNA - occur throughout the genome and are very common in cancer. CNAs can be simply detected by competitive PCR and melting curve analysis by limiting the dNTP concentration.

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High-Speed Melting
Melting rate, amplicon size, and analysis method affect the quality of small amplicon genotyping. Summarized in two publications, with optimal temperature control faster rates improve melting analysis, just like faster rates improve PCR.

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