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    REVIEWS, EDITORIALS, LETTERS, INTRODUCTIONS by Carl Wittwer
    1. Bustin SA, Ruijter JM, van den Hoff MJB, Kubista M, Pfaffl MW, Shipley GL, Tran N, Rödiger S, Untergasser A, Mueller R, Nolan T, Milavec M, Burns MJ, Huggett JF, Vandesompele J, Wittwer CT. (2025) MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines. Clin Chem. 2025 Apr 24;. doi: 10.1093/clinchem/hvaf043. PMID: 40272429.
    2. Wittwer CT, Zhou L, Ye F, Millington A, de Cola A, Kusukawa N. (2025) Making Molecular Diagnostics Faster. Int J Lab Hematol. 2025 Apr 22;. doi: 10.1111/ijlh.14487. PMID: 40260633.
    3. Wittwer CT, Hemmert AC, Kent JO, Rejali NA. (2024) DNA melting analysis. Mol Aspects Med. Jun;97:101268. doi: 10.1016/j.mam. PMID: 38489863.
    4. Lo YMD, Baudhuin LM, Pantel K, Chiu RWK, Nolte FS, Wittwer CT. (2020) Molecular Diagnostics: Going from Strength to Strength, Clin Chem 66;1-2.
    5. Bustin SA, Wittwer CT. (2017) MIQE: A step toward more robust and reproducible quantitative PCR. Clin Chem; 63:1537-8.
    6. Wittwer CT. (2017) Democratizing the Real-Time PCR. Clin Chem 63;924-5.
    7. Wittwer CT. (2016) Portable nanopore sequencing for viral surveillance. Clin Chem 62;1427-1429.
    8. Wittwer CT. (2011) Evolution of Polymerase Chain Reaction. Gen Eng News 31;38-39.
    9. Wittwer CT. (2010) Fully Automated Molecular Diagnostics: where are we and where are we going? Advance for Administrators of the Laboratory. Merion Publications, King of Prussia, PA 19;38-40.
    10. Wittwer CT. (2010) Making DNA melting useful. Clin Chem 56;1500-1.
    11. Montgomery J, Sandford L, and Wittwer CT. (2010) High-resolution DNA melting analysis. Exp Rev of Mol Diag 10:219-40.
    12. Wittwer CT. (2009) High resolution DNA melting: advances and limitations. Hum Mutat 30;857-859.
    13. Lo YM, Wittwer CT. (2009) Molecular diagnostics: at the cutting edge of translational research. Clin Chem 55;601.
    14. Wittwer CT. (2005) Book review of: DNA from A to Z, by Daniel H. Farkas. Washington, DC: AACC Press, 2004, 144 pp. Clin Chem 51;1320-1.
    15. Gingeras TR, Higuchi R, Kricka LJ, Lo YM, Wittwer CT. (2005) Fifty Years of Molecular analysis using (DNA/RNA) Diagnostics. Clin Chem. 51 (3):661-71.
    16. Wittwer CT. (2003) Book review of: RT-PCR Protocols. Methods in Molecular Biology, Vol. 193. Joe O'Connell, ed. Totowa, NJ: Humana Press, 2002, 378 pp. Clin Chem 49;345.
    17. Wittwer CT. (2002) Introduction to real-time PCR and other homogeneous methods for human DNA analysis, in Molecular Testing in Laboratory Medicine: Selections from Clinical Chemistry, 1998-2001, with Annotations and Updates. DE Bruns, YMD Lo, and CT Wittwer, eds., AACC press, Washington, DC, pp. 3-4.
    18. Wittwer CT. (2001) Introduction to rapid cycle real-time PCR: methods and applications, in Rapid Cycle Real-Time PCR. S Meuer, CT Wittwer, K Nakaguwara, eds, Springer, Berlin, pp.1-8.

    BOOK CHAPTERS
    1. Park J, Wittwer CT, Lo YMD, Chiu R. (2024) Genomes, Variants, and Massively Parallel Methods, in Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 9th ed. Rafai N, Chiu R, Young I, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 48.
    2. Rifai N, Chiu RWK, Wittwer CT, Young I. (2024) Clinical Chemistry and Molecular Diagnostics, in Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 9th ed. Rafai N, Chiu R, Young I, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 1.
    3. Genzen, JR, Hawker CD, Wittwer CT, Burnham CA. (2023) Automation in the clinical laboratory, in Tietz Textbook of Laboratory Medicine, 7th ed, Rafai N, Chiu R, Young I, Burnham CA, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 29.
    4. Tsongalis G, Wittwer CT. (2023) Nucleic acid techniques, in Tietz Textbook of Laboratory Medicine, 7th ed, Rafai N, Chiu R, Young I, Burnham CA, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 164.
    5. Park J, Wittwer CT. (2023) Genomes, variants and massively parallel methods. in Tietz Textbook of Laboratory Medicine, 7th ed, Rafai N, Chiu R, Young I, Burnham CA, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 65.
    6. Rafai N, Chiu R, Young, I Burnham CA, Wittwer CT. (2017) Laboratory medicine, in Tietz Textbook of Hawker CD, Genzen, JR, Wittwer CT. Automation in the clinical laboratory, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 6th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 26.
    7. Hawker CD, Genzen, JR, Wittwer CT. (2018) Automation, in Tietz Fundamentals of Clinical Chemistry, 8th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 16.
    8. Wittwer CT, Makrigioros GM. (2018) Molecular techniques, in Tietz Fundamentals of Clinical Chemistry, 8th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 48.
    9. Howe JG, Park J, Mardis ER, Wittwer CT. (2018) Molecular principles in Tietz Fundamentals of Clinical Chemistry, 8th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 47.
    10. Rafai N, Horvath AR, Wittwer CT. (2018) Clinical chemistry, molecular diagnostics, and laboratory medicine in Tietz Fundamentals of Clinical Chemistry, 8th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 1.
    11. Zhou L, Palais RA, Ardon Y, Wittwer CT. (2017) Competitive PCR for copy number assessment by restricting dNTPs, in Genomic Mosaicism in Neurons and Other Cell Types, Frada JM, Gage FH, eds. Springer, New York, Chapter 8, pp. 143-162.
    12. Hawker CD, Genzen, JR, Wittwer CT. (2017) Automation in the clinical laboratory, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 6th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 26.
    13. Wittwer CT, Makrigioros GM. (2017) Nucleic acid techniques, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 6th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 47.
    14. Wittwer CT, Park J. (2017) Genomes and variants. in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 6th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 45.
    15. Rafai N, Horvath AR, Wittwer CT. (2017) Clinical chemistry and molecular diagnostics, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 6th ed, Rafai N, Horvath AR, Wittwer CT, eds, Elsevier Science, Philadelphia, Chapter 1.
    16. Farrar, JS Wittwer CT. (2017) High resolution melting curve analysis for molecular diagnostics. In: Molecular Diagnostics, 3nd ed., Patrinos GP, Danielson P, Ansorge WJ, eds. Elsevier, London, Chapter 6, 79-102.
    17. Nolte FS, Wittwer CT. (2016) Nucleic acid amplification technology, in Molecular Microbiology: Diagnostic Principles and Practice, 3nd Ed. DH Persing et al, eds., ASM Press, Washington DC, Chapter 4.
    18. Wittwer CT, Kusukawa N. (2013) Genomes and Nucleic Acid Alterations, in Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 5th ed, Burtis C and Bruns DE, eds, Elsevier Science, Philadelphia, Chapter 49.
    19. Wittwer CT, Kusukawa N. (2013) Nucleic Acid Techniques and Applications, in Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 5th ed, Burtis C and Bruns DE, eds, Elsevier Science, Philadelphia, Chapter 48.
    20. Wittwer CT, Kusukawa N. (2012) Genomes and Nucleic Acid Alterations, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th ed, Burtis C, Ashwood ER, and Bruns DE, eds, Elsevier Science, Philadelphia, Chapter 38, 1225-1230.
    21. Wittwer CT, Kusukawa N. (2012) Nucleic Acid Techniques, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th ed, Burtis C, Ashwood ER, and Bruns DE, eds, Elsevier Science, Philadelphia, Chapter 17, 401-442.
    22. Zhou L, Smith D, Wittwer CT. (2012) Rare allele enrichment and detection by allele specific PCR, competitive probe blocking and melting analysis. BioTechniques Protocol Guide (DOI 10.2144/000113783).
    23. Dobrowolski SF and Wittwer CT. (2011) High Resolution Melt Profiling. In Molecular Analysis and Genome Discovery, 2nd Ed., Rapley R, and Harbron S, eds. John Wiley & Sons, Hoboken, NJ, Chapter 5, 81-113.
    24. Mackay JF, Wittwer CT. (2011) High resolution melting analysis, in PCR Optimization and Troubleshooting, Horizon Scientific Press, Norwich, UK, Chapter 10, 201-216.
    25. Wittwer CT and Farrar J. (2011) Magic in solution: An introduction and brief history of PCR, in PCR Optimization and Troubleshooting, Horizon Scientific Press, Norwich, UK, Chapter 1, 1-22.
    26. Wittwer CT and N Kusukawa. (2011) Real-Time PCR and Melting Analysis, in Molecular Microbiology: Diagnostic Principles and Practice, 2nd Ed. DH Persing, FC Tenover, R Hayden, F Nolte, YW Tang, AV Belkum, eds., ASM Press, Washington DC, Chapter 4, 63-82.
    27. McKinney JT, Nay LM, De Koeyer D, Reed GH, Wall M, Palais RA, Jarret RL, Wittwer CT. (2010) Mutation scanning and genotyping in plants by high resolution DNA melting. In: The Handbook of Plant Mutation Screening, Meksem K, Kahl G, eds. Wiley-VCH, Weinheim, 149-165.
    28. Farrar, JS, Reed GH, Wittwer CT. (2010) High resolution melting curve analysis for molecular diagnostics. In: Molecular Diagnostics, 2nd ed., Patrinos GP, Ansorge WJ, eds. Elsevier, London, Chapter 15, 229-245.
    29. Wittwer CT, Rasmussen RP, Ririe KM. (2010) Rapid PCR and melting curve analysis. In: The PCR Revolution: Basic Technologies and Applications, Bustin SA, ed. Cambridge Univ Press, New York, 48-69.
    30. Wittwer CT, N Kusukawa. (2007) Genomes and Nucleic Acid Alterations, in Fundamentals of Molecular Diagnostics, Bruns DE, Ashwood ER, Burtis CA, eds, Elsevier Science, Philadelphia, Chapter 2, 17-21.
    31. Wittwer CT, N Kusukawa.(2007) Nucleic Acid Techniques, in Fundamentals of Molecular Diagnostics, Bruns DE, Ashwood ER, Burtis CA, eds, Elsevier Science, Philadelphia, Chapter 5, 46-79.
    32. Dujols V, Kusukawa N, McKinney JT, Dobrowolsky SF, Wittwer CT. (2006) High-resolution melting analysis for scanning and genotyping., in Real Time PCR. Tevfik D, ed., Taylor and Francis, Abingdon, Chapter 9, 155-169.
    33. Pryor, R, CT Wittwer. (2006) Real Time PCR and Melting Curve Analysis. In: Clinical Applications of PCR (Methods in Molecular Biology), Lo YMD, Chiu RWK, and Chan KCA, eds. Humana Press, Totowa NJ, Chapter 3, 19-32.
    34. Wittwer CT, N Kusukawa. (2005) Nucleic Acid Techniques, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 4th ed, Burtis C, Ashwood ER, and Bruns DE, eds, Elsevier Science, Philadelphia, Chapter 37, 1407-1449.
    35. Wittwer CT, N Kusukawa. (2005) Molecular Diagnostics Technology, in Clinical Diagnostic Technology: The Total Testing Process. K Ward-Cook, C Lehmann, L Schoeff, and RH Williams, eds., AACC Press, Washington DC, Chapter 15, 341-369.
    36. Wittwer CT and N Kusukawa. (2004) Real-Time PCR, in Molecular Microbiology: Diagnostic Principles and Practice. DH Persing, FC Tenover, J Versalovic, YW Tang, ER Unger, DA Relman, TJ White, eds., ASM Press, Washington, DC, 71-84.
    37. Chang CC, S Perkins and CT Wittwer. (2002) Measurement of MDR1 gene expression by real-time quantitative RT-PCR using the LightCycler instrument. In: Rapid Cycle Real-Time PCR – Methods and Applications: Genetics and Oncology. Dietmaier, C. Wittwer, and Sivasubrananian, (eds), Springer, Berlin, 199-205.
    38. Gundry CN and CT Wittwer. (2002) SYBR Green I analysis of the trinucleotide repeat responsible for Huntington’s disease. In: Rapid Cycle Real-Time PCR – Methods and Applications: Genetics and Oncology. Dietmaier, C. Wittwer, and Sivasubrananian, (eds), Springer, Berlin, 57-63.
    39. Wittwer CT, PS Bernard, AO Crockett, SD Bohling and KSJ Elenitoba-Johnson. (2002) Single nucleotide polymorphism (SNP) genotyping by probe melting curve analysis, in Tumor Markers: Physiology, Pathobiology, Technology and Clinical Applications. EP Diamandis, HA Fritsche, H Lilja, DW Chan and MK Schwartz, ed., AACC press, Washington, DC, 513-515.
    40. Wittwer CT and MG Herrmman. (1999) Rapid thermal cycling and PCR kinetics, in PCR Methods Manual (Innis M, D Gelfand, and J. Sninsky, eds.), Academic Press, San Diego, 211-229.
    41. Wittwer CT, K Ririe, R. Rasmussen. (1998) Fluorescence monitoring of rapid cycle PCR for quantification, in Gene Quantification, Ferre, F., ed., Birkhauser, New York, 129-144.
    42. Brown RA, MJ Lay, and CT Wittwer. (1998) Rapid cycle amplification for construction of competitive templates, in Genetic Engineering with PCR, (Horton, RM and RC Tait, eds.), Horizon Scientific Press, Norfolk, England, 57-70.
    43. Wittwer, CT, GB Reed and KM Ririe. (1994) Rapid cycle DNA amplification. In K Mullis, F Ferre and R Gibbs (Eds.), The polymerase chain reaction. Springer-Verlag, Deerfield Beach, FL, 174-181.
    44. Wittwer CT, BW Wyse, RG Hansen. (1986) Enzymatic hydrolysis of pantetheine, in: Methods in Enzymology 122:36-43.
    45. Wittwer CT, BW Wyse, RG Hansen. (1985) Pantetheine, in: HU Bergmeyer, ed., Methods of Enzymatic Analysis, 3rd Ed., Volume VII, 244-249, Verlag Chemie, Weinheim.
    BOOK
    1. N Rifai, AR Horvath, CT Wittwer, A Hoofnagle (2018) Principles and applications of clinical mass spectrometry: small molecules, peptides, and pathogens. Elsevier. https://doi.org/10.1016/C2017-0-03476-6. ISBN 978-0-12-816063-3

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