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| Speed of PCR Publications |
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In less than a minute, the entire PCR process can be completed: from the pre-PCR step (sample prep), to amplification, and post-PCR analysis (melting analysis). Below are our papers on this topic.
- Extreme PCR (PCR in less than a minute)
- High-speed melting analysis (in 1 to 4 seconds)
Pryor RJ et. al. (2017) High-Speed Melting Analysis: The Effect of Melting Rate on Small Amplicon Microfluidic Genotyping. Clin Chem. PMID: 28818830.
- Fast sample preparation with NaOH (in < 5 seconds)
Wittwer CT et al. (2025) Making Molecular Diagnostics Faster. Int J Lab Hemtaol. PMID: 40260633. Data shown as part of this review
- Integrated PCR process (in less than 1 minute)
Myrick JT et. al. (2019) Integrated Extreme Real-Time PCR and High-Speed Melting Analysis in 52 to 87 Seconds. Clin Chem. PMID: 30459167
REVIEWS
- Wittwer CT et al. (2025) Making Molecular Diagnostics Faster. Int J Lab Hemtaol. PMID: 40260633.
Others have also succeeded in speeding up PCR. Reproduced here is the supplemental table of speeds reported at 10 seconds per PCR cycle or faster. We compared those with extreme PCR (clocked at 0.42 seconds per cycle in our lab), and our 1990 first-generation commercial instrument Rapid Cycler (20 seconds per cycle).
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Wittwer CT et al. (2024) DNA Melting Analysis. Mol Aspects Med. PMID: 38489863. Review.
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