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Building High-Resolution Melting


This is photo archive of instruments developed by the Wittwer Lab and collaborators. Many of the commercial instruments are no longer in production and reside in company galleries and labs of early customers.

First Prototype - Experimental single sample high-resolution melting instrument. Wittwer Lab & Idaho Technology R&D (2002).




HR-1: First generation commercial instrument. Idaho Technology (2003) - To this day, this instrument produces the most precise DNA melting data at the highest resolution. Even though this is a one sample instrument, it collects about 200 datapoints per °C, and its superior raw data quality is hard to beat. No data smoothing is necessary for analysis. Melting is complete in 2 min or less. No longer in production.




LightScanner: Second generation commercial instrument based on 96- or 384-well plates. Idaho Technology (2005) - Since the introduction of this instrument, other companies have followed suit with multi-sample high-resolution melting systems. For many years, only two other instruments had acceptable performance, with data density of about 10 datapoints per °C during the melting operation (Herrmann et al 2007). More instruments with reasonable performance are now available. No longer in production.

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