Summary
The Tm ToolSM is a web-based application that calculates and predicts the melting temperature of DNA (primers, probes, and small oligos). Knowing the melting temperature of oligos is an essential step to optimizing assay design. This tool calculates Tm with the nearest-neighbor method with adjustments for laboratory conditions such as [Mg++] and sequence concentrations.
What is Tm?
The melting temperature is defined as the temperature in which 50% of the DNA strands are in the helix state and the other 50% in the coil state (single stranded).