A Bit of History...
New to Melting? Watch the High-Resolution Melting Webinar with Dr. Carl Wittwer (Biotechniques, 2017)
High-resolution melting analysis is a novel method introduced in 2003. Similar to the LightCycler, this too was a result of collaboration between our lab at the University of Utah and Idaho Technology, Inc (Salt Lake City, UT). The method uses high data-density acquisition, and detects small sequence differences in PCR fragments, just by direct melting. Melting curves can now be used for mutation scanning, sequence matching, and mutliplex genotyping - analyses that traditionally required processing of PCR products by electrophoresis or other non-homogeneous means. Because of its speed and simplicity, popularity of this method is growing.
Two inventions were key to high-resolution melting: 1) high-resolution instruments, and 2) novel saturation dyes. To read more, please follow the colored tabs in the navigation bar (top of page) ..
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